Batter and Scout

Batter is from OFF, Scout is from Team Fortress 2.

Someone else noticed ^

Hayate and Tamahome

Hayate is from Pretear, Tamahome is from Fushigi Yuugi.

Jean Kirstein and Vato Falman

Jean Kirstein is from Attack on Titan, Vato Falman is from Fullmetal Alchemist.

Rose Lalonde and Courtney Crumrin

Rose Lalonde is from Homestuck, Courtney is from Courtney Crumrin and the Night Things.

Sakura Haruno and Akane Motomiya

Sakura Haruno is from Naruto, Akane Motomiya is from Harukanaru Toki no Naka de.

Billie Joe Armstrong, Dero and RayWilliamJohnson

Billie Joe Armstrong is from Green Day, Dero is from Oomph and Ray is from raywilliamjohnson.

Toshiro Hitsugaya and Mannen

Toshiro Hitsugaya is from Bleach, Mannen is from Pretear.

Bakura Ryou and Mytho

Bakura Ryou is from Yu-Gi-Oh, Mytho is from Princess Tutu.

Kyoya Ootori, Sasame and Peter White

Kyoya is from Ouran Highschool Host Club, Sasame is from Pretear and Peter is from Heart no Kuni no Alice.

Lucy Heartfilia, Winry Rockbell and Misa Amane

Lucy is from Fairy Tail, Winry is from Fullmetal Alchemist, Misa is from Death Note.

Sakura Kinomoto and Miaka Yuki

Sakura is from Cardcaptor Sakura, Miaka is from Fushigi Yuugi.

Chell and Zoey

Chell from Portal, Zoey from Left 4 Dead

Konata Izumi and Reverie Metherlence

Konata is from Lucky Star, Reverie is from Elemental Gelade.

Laharl and Chrono

Laharl is from Disgaea, Chrono is from Chrono Crusade.

Kyo Sohma and Tenma Morimura

Kyo is from Fruits Basket, Tenma is from Harukanaru Toki no Naka De.

Winner Sinclair and Tamaki Suoh

Tamaki is from Ouran Highschool Host Club, Winner is from Karin

Haninozuka Mitsukuni and Momiji Sohma

Mitsukuni is from Ouran Highschool Host Club, Momiji is from Fruit's Basket.